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Liquid Chromatograph

About Chromatography

Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures. It is a chemical technique for separating mixtures of coloured chemicals. This technique is important in biology as well as chemistry. There are a number of different types of chromatography in use, including gas, liquid, paper, and gel permeation chromatography.

Modern scientific instruments, such as spectrometers, chromatographs, and microscopes, generate data in electronic formats using integrated or external data acquisition and processing systems. Older equipment has (or can be retrofitted with) serial ports to transfer data to external computers.

The data fields provided by analytical instruments often include technician and sample details, operational parameters, and analyst's comments, in addition to the technical data (e.g., spectrum, chromatogram, and image).

High performance liquid chromatography (or high pressure liquid chromatography, HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry to separate, identify, and quantify compounds based on their idiosyncratic polarities and interactions with the column's stationary phase.

The use of smaller particle size column packing (which creates higher backpressure) increases the linear velocity giving the components less time to diffuse within the column, leading to improved resolution in the resulting chromatogram. Common solvents used include any miscible combination of water or various organic liquids (the most common are methanol and acetonitrile). This partitioning process is similar to that which occurs during a liquid-liquid extraction but is continuous, not step-wise.

Gas chromatography-mass spectrometry (GC-MS) is a method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample. The GC-MS has been widely heralded as a "gold standard" for forensic substance identification because it is used to perform a specific test.

The GC-MS is composed of two major building blocks the gas chromatograph and the mass spectrometer. The gas chromatograph utilizes a capillary column which depends on the column's dimensions (length, diameter, film thickness) as well as the phase properties (e.g. 5% phenyl polysiloxane).

Our portfolio of gas chromatograph products includes:

- At-line gas chromatographs
- Laboratory gas chromatographs
- Process gas chromatographs
- Gas chromatograph sample handling systems
- Liquid sample injection valves and continuous performance sliding plate valves.

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